Installing Post-translational Modifications Site-Specifically using GCE
Date: Monday, July 28 thru Friday, August 1, 2025
Location: GCE4-All Research Center, Oregon State University, Corvallis, Oregon, USA
The 2025 GCE4All Center Workshop will focus on the theory and practice of installing site-specifically non-canonical amino acids (ncAAs) that represent post-translational modifications (PTMs) in proteins. Academic and industrial attendees are welcome and will receive hands-on training from Center scientists on how to express, purify, and characterize recombinant proteins containing site-specifically installed PTMs, including phospho-serine, non-hydrolyzable phospho-serine, phospho-threonine, acetyl-lysine, 3-nitro-tyrosine, and halogenated tyrosines. All attendees will perform control protein expressions in E. coli with plasmids and ncAAs provided by the Center. In addition, academic attendees can bring to the workshop a gene of their choice cloned ahead of time into a specified expression vector with encoded TAG sites for ncAA installation. A series of lectures will discuss the practice, theory, history, strengths and challenges of Genetic Code Expansion as it relates to installing PTMs and characterizing PTM-proteins. Attendees are encouraged to apply early so that they can discuss with Center scientists prior to the Workshop the protein and PTMs they would like to encode and feasibility of success.