Bioorthogonal Ligations-Clicking with GCE
Date: August 8-12, 2023
Location: GCE4-All Research Center, Oregon State University, Corvallis, Oregon, USA
Applications open in March
Advances in Genetic Code Expansion and bioorthogonal ligations have changed the landscape of protein studies by enabling site-specific conjugation to proteins in vitro and in vivo. Genetically encoding bioorthogonal ligations is inherently a fusion of synthetic biology and chemical biology. Success requires 1) the addition of all the necessary translational components to an organism that allow it to efficiently produce a protein with new chemical functionality site-specifically encoded, 2) optimization of scientific studies of the chemically modified protein in vivo or in vitro, and 3) optimization of the labeling reaction to the protein of interest while minimizing any background reactions. Overall, success with genetically encoding bioorthogonal ligations relies on proficiency in both synthetic biology and chemical biology.