Date: Monday, July 28 thru Friday, August 1, 2025
Location: GCE4-All Research Center, Oregon State University, Corvallis, Oregon, USA
Please fill out our GCE Interest Survey to describe your training needs:
ACCESS SURVEY HERE
Application Information:
- Application vs. Registration: Completing an application will not register you to attend, nor does it guarantee acceptance to attend. Registration can only be completed once you have been accepted by the Workshop Chair/Committee. If accepted, you will be notified via email with an unpublished link to register for the workshop. Registration and payment arrangements are expected at that time.
- Payments: Payment in full (less any financial support awarded) is required at the time of registration.
- Financial Aid: We understand that your acceptance is sometimes conditional on the amount of financial aid provided to you. Note, at this time financial aid is only available to support workshop participants.
- Wait List: We anticipate the workshop to be full before the deadline. If the workshop is oversubscribed you will be placed on a waiting list. Applications will be considered at the discretion of the workshop chairs. One application per individual.
Important Dates:
- Acceptances will be announced on a rolling basis via email.
- Applications will be reviewed weekly with acceptances/rejections sent via email.
- Early bird registration ends on May 15, 2025. Once accepted we ask that you register early to lock in reduced prices and secure your spot!
Workshop Application – Financial Aid Request:
Full scholarship and half scholarship support is available for the GCE workshop. The number of scholarships available depends on a number of factors, including the level of financial support in a given year and the number of students requesting stipends. Scholarships are awarded to support those historically underrepresented in STEM research and education.
Course Fee:
Early Academic – $2,500 (Until May 15, 2025)
Late Academic – $2,800 (After May 15, 2025)
Early Industry – $4,500 (Until May 15, 2025)
Late Industry – $5,000 (After May 15, 2025)
Description:The 2025 GCE4All Center Workshop will focus on the theory and practice of installing site-specifically non-canonical amino acids (ncAAs) that represent post-translational modifications (PTMs) in proteins. Academic and industrial attendees are welcome and will receive hands-on training from Center scientists on how to express, purify, and characterize recombinant proteins containing site-specifically installed PTMs, including phospho-serine, non-hydrolyzable phospho-serine, phospho-threonine, acetyl-lysine, 3-nitro-tyrosine, and halogenated tyrosines. All attendees will perform control protein expressions in E. coli with plasmids and ncAAs provided by the Center. In addition, academic attendees can bring to the workshop a gene of their choice cloned ahead of time into a specified expression vector with encoded TAG sites for ncAA installation. A series of lectures will discuss the practice, theory, history, strengths and challenges of Genetic Code Expansion as it relates to installing PTMs and characterizing PTM-proteins. Attendees are encouraged to apply early so that they can discuss with Center scientists prior to the Workshop the protein and PTMs they would like to encode and feasibility of success.
Lectures:
- GCE Overview and status of the field
- Fundamentals of post-translation modifications with GCE
- Overview of ncAA classes & categories
- Plasmid architecture and cloning
- Adopting GCE for biological proteins
- Good, bad, the ugly – and the future of GCE
- Translation biology